Psychoactive Plant Database - Neuroactive Phytochemical Collection

1. Sci Rep. 2024 Nov 4;14(1):26600. doi: 10.1038/s41598-024-76247-7.

Author Correction: Myricitrin Alleviates Oxidative Stress-induced Inflammation 
and Apoptosis and Protects Mice against Diabetic Cardiomyopathy.

Zhang B(1)(2)(3)(4), Shen Q(5), Chen Y(6), Pan R(1), Kuang S(7), Liu G(8), Sun 
G(9)(10)(11)(12), Sun X(13)(14)(15)(16).

Author information:
(1)Institute of Medicinal Plant Development, Peking Union Medical College and 
Chinese Academy of Medical Sciences, Beijing, 100193, China.
(2)Key Laboratory of Bioactive Substances and Resources Utilization of Chinese 
Herbal Medicine, Ministry of Education, Beijing, 100193, China.
(3)Beijing Key Laboratory of Innovative Drug Discovery of Traditional Chinese 
Medicine (Natural Medicine) and Translational Medicine, Beijing, 100193, China.
(4)Key Laboratoryof efficacy evaluation of Chinese Medicine against glyeolipid 
metabolism disorder disease, State Administration of Traditional Chinese 
Medicine, Beijing, 100193, China.
(5)Center of Research and Development on Life Sciences and Environmental 
Sciences, Harbin University of Commerce, Harbin, 150076, China.
(6)School of Life Science, Beijing Institute of Technology, Beijing, 100081, 
China.
(7)Department of Animal Sciences, Purdue University, West Lafayette, IN, 47907, 
USA.
(8)School of Life Science, Beijing Institute of Technology, Beijing, 100081, 
China. gyliu@bit.edu.cn.
(9)Institute of Medicinal Plant Development, Peking Union Medical College and 
Chinese Academy of Medical Sciences, Beijing, 100193, China. sunguibo@126.com.
(10)Key Laboratory of Bioactive Substances and Resources Utilization of Chinese 
Herbal Medicine, Ministry of Education, Beijing, 100193, China. 
sunguibo@126.com.
(11)Beijing Key Laboratory of Innovative Drug Discovery of Traditional Chinese 
Medicine (Natural Medicine) and Translational Medicine, Beijing, 100193, China. 
sunguibo@126.com.
(12)Key Laboratoryof efficacy evaluation of Chinese Medicine against glyeolipid 
metabolism disorder disease, State Administration of Traditional Chinese 
Medicine, Beijing, 100193, China. sunguibo@126.com.
(13)Institute of Medicinal Plant Development, Peking Union Medical College and 
Chinese Academy of Medical Sciences, Beijing, 100193, China. 
sun_xiaobo163@163.com.
(14)Key Laboratory of Bioactive Substances and Resources Utilization of Chinese 
Herbal Medicine, Ministry of Education, Beijing, 100193, China. 
sun_xiaobo163@163.com.
(15)Beijing Key Laboratory of Innovative Drug Discovery of Traditional Chinese 
Medicine (Natural Medicine) and Translational Medicine, Beijing, 100193, China. 
sun_xiaobo163@163.com.
(16)Key Laboratoryof efficacy evaluation of Chinese Medicine against glyeolipid 
metabolism disorder disease, State Administration of Traditional Chinese 
Medicine, Beijing, 100193, China. sun_xiaobo163@163.com.

Erratum for
    Sci Rep. 2017 Mar 13;7:44239. doi: 10.1038/srep44239.

DOI: 10.1038/s41598-024-76247-7
PMCID: PMC11535346
PMID: 39496652


2. J Nat Med. 2024 Oct 29. doi: 10.1007/s11418-024-01856-5. Online ahead of
print.

Exploring the inhibitory activity and mechanism on lipid production in 3T3-L1 
cells by hot water extract derived from Acacia confusa flowers.

Tsao NW(1), Cheng JY(2), Wang SY(3)(4)(5).

Author information:
(1)Department of Forestry, National Chung-Hsing University, 250 Kuo-Kuang Road, 
Taichung, 402, Taiwan.
(2)Program in Special Crop and Metabolome, Academy of Circle Economy, National 
Chung Hsing University, Nantou, 540, Taiwan.
(3)Department of Forestry, National Chung-Hsing University, 250 Kuo-Kuang Road, 
Taichung, 402, Taiwan. taiwanfir@dragon.nchu.edu.tw.
(4)Program in Special Crop and Metabolome, Academy of Circle Economy, National 
Chung Hsing University, Nantou, 540, Taiwan. taiwanfir@dragon.nchu.edu.tw.
(5)Agricultural Biotechnology Research Center, Academia Sinica, Taipei, 108, 
Taiwan. taiwanfir@dragon.nchu.edu.tw.

Acacia confusa Merr. (Fabaceae) (A. confusa) is a native tree species of Taiwan, 
commonly found in the low-altitude mountains and hilly areas of the Hengchun 
Peninsula. This evergreen, perennial, and large-sized tree was the focus of a 
study that employed various chromatographic and spectroscopic methods to analyze 
the hot water extract of its flowers. The analysis revealed that the major 
components of the extract were myricitrin, quercitrin, europetin-3-O-rhamnoside, 
and chalconaringenin-2'-xyloside, with respective concentrations of 
approximately 0.22, 0.02, 0.26, and 0.10 mg/g of the flowers. Subsequent cell 
assays were conducted to assess the inhibitory effect of the extract on lipid 
synthesis in fat cells. Oil Red O staining results indicated that the extract 
significantly suppressed fatty acid accumulation in 3T3-L1 cells, with the most 
pronounced effect observed at a concentration of 180 μg/ml. Furthermore, the hot 
water extract of A. confusa flowers was found to increase the phosphorylation of 
AMP-activated protein kinase (AMPK), decrease the phosphorylation of cAMP 
response element-binding protein (CREB), and reduce the expression of 
glucocorticoid receptor (GR) protein. This, in turn, inhibited the expression of 
downstream transcription factors such as CCAT/ehancer binding proteins α 
(C/EBPα), CCAT/ehancer binding proteins β (C/EBPβ), CCAT/ehancer binding 
proteins δ (C/EBPδ), peroxisome proliferation-actived receptor γ (PPARγ), and 
sterol regulatory element binding proteins-1 (SREBP-1). Consequently, the 
expression of lipid synthesis-related proteins acetyl-CoA carboxylase (ACC), 
fatty acid synthase (FAS), and fatty acid translocase (CD36) was reduced, 
ultimately inhibiting lipid generation. Therefore, the hot water extract of A. 
confusa flowers shows potential for development as a weight-loss tea.

© 2024. The Author(s) under exclusive licence to The Japanese Society of 
Pharmacognosy.

DOI: 10.1007/s11418-024-01856-5
PMID: 39470961


3. Molecules. 2024 Oct 11;29(20):4800. doi: 10.3390/molecules29204800.

Methanol Extract of Thottea siliquosa (Lam.) Ding Hou Leaves Inhibits 
Carrageenan- and Formalin-Induced Paw Edema in Mice.

Renny A(1), Sidhic J(2), Tom A(1), Kuttithodi AM(1), Job JT(1), Rajagopal R(3), 
Alfarhan A(3), Narayanankutty A(1).

Author information:
(1)Division of Cell and Molecular Biology, PG & Research Department of Zoology, 
St. Joseph's College (Autonomous), Calicut (Affiliated to University of Calicut) 
673008, India.
(2)Phytochemistry and Pharmacology Division, PG & Research Department of Botany, 
St. Joseph's College (Autonomous), Calicut 673008, India.
(3)Department of Botany and Microbiology, College of Science, King Saud 
University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.

Inflammation is a physiological condition that when unattended causes serious 
health concerns over the long term. Several phytocompounds have emerged as 
promising sources of anti-inflammatory agents. Thottea siliquosa is a 
traditional medicine for inflammatory and toxicity insults; however, this has 
not been scientifically confirmed. The purpose of this study is to evaluate the 
anti-inflammatory properties of T. siliquosa methanol leaf extract in a mouse 
model. This study investigates the anti-inflammatory activities of a plant 
extract obtained from leaves of T. siliquosa (TSE) with a focus on carrageenan- 
and formalin-induced paw oedema in mice. The extract's efficacy was assessed 
using well-established inflammation models, and the results showed a 
considerable reduction in paw edema in both cases. In the case of carrageenan 
model TSE at 50 mg/kg showed a 53.0 ± 2.5% reduction in edema, while those 
treated with TSM at 100 mg/kg exhibited a 60.0 ± 1.8% reduction (p < 0.01). In 
the case of a formalin model when a higher dose of TSE (100 mg/kg) was given, 
paw thickness decreased by 47.04 ± 1.9% on the fifth day and by 64.72 ± 2.2% on 
the tenth day. LC-MS analysis reported the presence of gallic acid, quinic acid, 
quercetin, clitorin, myricitrin, retronecine, batatasin II, gingerol, and 
coumaric acid in the extract. Overall, this study confirms that T. siliquosa 
extract exerts anti-inflammatory effects in animals and is possibly mediated 
through the combined effects of these phytochemicals.

DOI: 10.3390/molecules29204800
PMCID: PMC11510445
PMID: 39459169 [Indexed for MEDLINE]

Conflict of interest statement: The authors declare no conflicts of interest.


4. Biochem Biophys Res Commun. 2024 Nov 19;734:150771. doi: 
10.1016/j.bbrc.2024.150771. Epub 2024 Sep 30.

Myricetin and myricitrin indirectly and directly increases uncoupling protein-1 
mRNA expression in C3H10T1/2 beige adipocytes.

Takahashi H(1), Morimoto H(1), Tanaka M(1), Inoue H(1), Goto T(2), Kawada T(2), 
Uehara M(1), Takahashi N(3).

Author information:
(1)Laboratory of Physiology and Metabolism, Department of Nutritional Science 
and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture, 
Tokyo, Japan.
(2)Division of Food Science and Biotechnology, Graduate School of Agriculture, 
Kyoto University, Kyoto, Japan.
(3)Laboratory of Physiology and Metabolism, Department of Nutritional Science 
and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture, 
Tokyo, Japan. Electronic address: nt204885@nodai.ac.jp.

In thermogenic brown and beige adipocytes, the proton gradient formed by energy 
derived from nutrients such as lipids and carbohydrates is consumed by 
uncoupling protein-1 (UCP-1), resulting in thermogenesis without ATP production 
in the mitochondria. Accordingly, increased UCP-1 expression represents a 
crucial aspect of dietary management for individuals with overweight and 
obesity. Myricetin and its glycoside, myricitrin, are food-derived flavonoids 
that possess various beneficial effects. This is the first study to examine the 
effects of myricetin and myricitrin on the inflammation-inhibited expression of 
Ucp-1 using a modified cell-based assay with conditioned medium (CM). The CM 
derived from lipopolysaccharide (LPS)-activated RAW264.7 macrophages was 
observed to inhibit the Ucp-1 expression induced by adrenergic stimulation in 
10T1/2 adipocytes. Conversely, the CM derived from activated macrophages treated 
with myricetin or myricitrin reversed this inhibition of Ucp-1 expression. 
Subsequently, the direct effects of both the compounds on basal and 
adrenaline-induced expression of Ucp-1 were investigated. In contrast to a 
previous report, myricetin and myricitrin did not increase the basal Ucp-1 mRNA 
expression in 10T1/2 adipocytes when treated during the 
differentiation-promoting period. However, we have found for the first time that 
both compounds enhanced the adrenergic sensitivity of 10T1/2 adipocytes when 
treated during the differentiation-inducing period. These results indicate that 
myricetin and myricitrin have indirect effects on inflammation-induced 
suppression and direct effects on adrenergic sensitivity, suggesting a novel 
mechanism that both compounds increase Ucp-1 expression in vivo by both indirect 
and direct effects, rather than by affecting basal expression.

Copyright © 2024 Elsevier Inc. All rights reserved.

DOI: 10.1016/j.bbrc.2024.150771
PMID: 39369543 [Indexed for MEDLINE]

Conflict of interest statement: Declaration of competing interest The authors 
declare no financial or personal relationships that may be considered potential 
competing interests.


5. Chem Biodivers. 2024 Sep 24:e202402139. doi: 10.1002/cbdv.202402139. Online 
ahead of print.

Phytochemical Investigation of an Ostrya carpinifolia L. Extract: an Effective 
Anti-Pollution Cosmetic Active Ingredient.

Trinel M(1), Dubois C(1), Burger P(2), Plainfossé H(3), Azoulay S(4), 
Verger-Dubois G(5), Fernandez X(6).

Author information:
(1)Institut de Chimie de Nice, MVBV, 28 avenue valrose, 06100, Nice, FRANCE.
(2)Nissactive, r&d, Espace Jacques-Louis Lions, 4 traverse Dupont, 06130, 
GRASSE, FRANCE.
(3)Nissactive, R&D, Espace Jacques-Louis Lions, 4 traverse Dupont, 06130, Nice, 
FRANCE.
(4)Institut de Chimie de Nice, chimie, 28 avenue valrose, 06100, Nice, FRANCE.
(5)Nissactive, PDG, Espace Jacques-Louis Lions, 4 traverse Dupont, 06130, Nice, 
FRANCE.
(6)Institut de Chimie de Nice, MVBV, 28 avenue Valrose, 06108, Nice, FRANCE.

Ostrya carpinifolia L., a member of the Betulaceae family, is a tree endemic to 
the Mediterranean basin that is well known for the hardness of its wood. In this 
study, we assess the anti-pollution activities of a hydroalcoholic extract of O. 
carpinifolia twigs using several judiciously selected in vitro cosmetic 
bioassays. The extract's capacity to counteract excessive production of reactive 
oxygen species following a cutaneous exposure to atmospheric pollution was 
evaluated using a combination of several antioxidant assays: DPPH, FRAP and 
β-carotene bleaching assays. These antioxidant assays were complemented by 
anti-elastase, anti-collagenase, anti-hyaluronidase and anti-lipoxygenase assays 
to evaluate the capacity of the extract to preserve the integrity of the skin. 
The hydroalcoholic extract of O. carpinifolia demonstrates intriguing biological 
antioxidant activities, with approximately 50% inhibition observed in DPPH and 
β-carotene assays. Furthermore, its anti-lipoxygenase, anti-hyaluronidase, and 
anti-collagenase activities are noteworthy, exceeding 50% inhibition. The two 
major compounds of O. carpinifolia ethanolic extract were isolated and 
identified as myricitrin (1) and quercitrin (2). Myricitrin and quercitrin 
exhibit antioxidant and anti-hyaluronidase properties; we explored the 
correlation of these properties with the activity of the crude hydroalcoholic 
extract. Notably, these compounds have not been previously described in the 
Ostrya genus.

© 2024 Wiley‐VCH GmbH.

DOI: 10.1002/cbdv.202402139
PMID: 39316583