Psychoactive Plant Database - Neuroactive Phytochemical Collection

1. Plant Physiol Biochem. 2024 Oct 22;217:109230. doi: 
10.1016/j.plaphy.2024.109230. Online ahead of print.

Arbuscular mycorrhizal symbiosis enhances the accumulation of plant-derived 
carbon in soil organic carbon by regulating the biosynthesis of plant 
biopolymers and soil metabolism.

Liu Y(1), Qian J(2), Lu B(3), Hu J(4), He Y(3), Shen J(3), Tang S(3).

Author information:
(1)Key Laboratory of Integrated Regulation and Resource Development on Shallow 
Lakes, Ministry of Education, Hohai University, 210098, Nanjing, People's 
Republic of China; College of Environment, Hohai University, 210098, Nanjing, 
People's Republic of China. Electronic address: 1650026514@qq.com.
(2)Key Laboratory of Integrated Regulation and Resource Development on Shallow 
Lakes, Ministry of Education, Hohai University, 210098, Nanjing, People's 
Republic of China; College of Environment, Hohai University, 210098, Nanjing, 
People's Republic of China. Electronic address: hhuqj@hhu.edu.cn.
(3)Key Laboratory of Integrated Regulation and Resource Development on Shallow 
Lakes, Ministry of Education, Hohai University, 210098, Nanjing, People's 
Republic of China; College of Environment, Hohai University, 210098, Nanjing, 
People's Republic of China.
(4)Department of Civil, Environmental and Construction Engineering, University 
of Central Florida, 32816, Orlando, Fl, USA.

Plant-derived carbon (C) is a critical constituent of particulate organic carbon 
(POC) and plays an essential role in soil organic carbon (SOC) sequestration. 
Yet, how arbuscular mycorrhizal fungi (AMF) control the contribution of 
plant-derived C to SOC storage through two processes (biosynthesis of plant 
biopolymers and soil metabolism) remains poorly understood. Here, we utilized 
transcriptome analysis to examine the effects of AMF on P. communis roots. Under 
the AM symbiosis, root morphological growth and tolerance to stress were 
strengthened, and the biosynthetic pathways of key plant biopolymers (long-chain 
fatty acids, cutin, suberin, and lignin) contributing to the plant-derived C 
were enhanced. In the subsequent metabolic processes, AMF increased soil 
metabolites contributing to plant-derived C (such as syringic acid) and altered 
soil metabolic pathways, including carbohydrate metabolism. Additionally, 
C-acquiring soil extracellular enzyme activities were enhanced by AMF, which 
could affect the stabilization of plant-derived C in soil. The contents of POC 
(21.71 g kg-1 soil), MAOC (10.75 g kg-1 soil), and TOC (32.47 g kg-1 soil) in 
soil were significantly increased by AMF. The concentrations of plant-derived C 
and microbial-derived C were quantified based on biomarker analysis. AMF 
enhanced the content of plant-derived C in both POC and MAOC fractions. What's 
more, plant-derived C presented the highest level in the POC fraction under the 
AMF treatment. This research broadens our understanding of the mechanism through 
which plant-derived C contributes to the accumulation of POC and SOC induced by 
AM symbiosis, and evidences the benefits of AMF application in SOC 
sequestration.

Copyright © 2024 Elsevier Masson SAS. All rights reserved.

DOI: 10.1016/j.plaphy.2024.109230
PMID: 39461054

Conflict of interest statement: Declaration of competing interest We declare 
that we have no known competing financial and personal relationships with other 
people or organizations that can inappropriately influence our work, there is no 
professional or other personal interest of any nature or kind in any product, 
service and/or company that could be construed as influencing the position 
presented in this manuscript.


2. Gels. 2024 Sep 30;10(10):631. doi: 10.3390/gels10100631.

BioMOF@cellulose Glycerogel Scaffold with Multifold Bioactivity: Perspective in 
Bone Tissue Repair.

Rosado A(1), Borrás A(1), Sánchez-Soto M(2), Labíková M(3)(4), Hettegger 
H(3)(5), Ramírez-Jiménez RA(6)(7), Rojo L(6)(7), García-Fernández L(6)(7), 
Aguilar MR(6)(7), Liebner F(3), López-Periago AM(1), Ayllón JA(8), Domingo C(1).

Author information:
(1)Institut de Ciència de Materials de Barcelona (ICMAB), Consejo Superior de 
Investigaciones Científicas (CSIC), Campus UAB s/n, 08193 Bellaterra, Spain.
(2)Departament de Ciència i Enginyeria de Materials, Escola d'Enginyeria de 
Barcelona Est (EEBE), Universitat Politècnica de Catalunya-Barcelona Tech (UPC), 
08019 Barcelona, Spain.
(3)Institute of Chemistry of Renewable Resources, University of Natural 
Resources and Life Sciences, Vienna (BOKU), Konrad-Lorenz-Strasse 24, A-3430 
Tulln an der Donau, Austria.
(4)Department of Organic Chemistry, University of Chemistry and Technology, 
Prague (UCT), Technická 5, 160 00 Praha 6-Dejvice, Czech Republic.
(5)Christian Doppler Laboratory for Cellulose High-Tech Materials, University of 
Natural Resources and Life Sciences, Vienna (BOKU), Konrad-Lorenz-Strasse 24, 
A-3430 Tulln an der Donau, Austria.
(6)Instituto de Ciencia y Tecnología de Polímeros (ICTP-CSIC), C/Juan de la 
Cierva, 3, 28006 Madrid, Spain.
(7)Networking Biomedical Research Centre in Bioengineering, Biomaterials and 
Nanomedicine (CIBER-BBN), Av. Monforte de Lemos, 3-5, 28029 Madrid, Spain.
(8)Departament de Química, Universitat Autònoma de Barcelona (UAB), Campus UAB 
s/n, 08193 Bellaterra, Spain.

The development of new biomaterials for musculoskeletal tissue repair is 
currently an important branch in biomedicine research. The approach presented 
here is centered around the development of a prototypic synthetic glycerogel 
scaffold for bone regeneration, which simultaneously features therapeutic 
activity. The main novelty of this work lies in the combination of an open meso 
and macroporous nanocrystalline cellulose (NCC)-based glycerogel with a fully 
biocompatible microporous bioMOF system (CaSyr-1) composed of calcium ions and 
syringic acid. The bioMOF framework is further impregnated with a third 
bioactive component, i.e., ibuprofen (ibu), to generate a multifold bioactive 
system. The integrated CaSyr-1(ibu) serves as a reservoir for bioactive 
compounds delivery, while the NCC scaffold is the proposed matrix for cell 
ingrowth, proliferation and differentiation. The measured drug delivery 
profiles, studied in a phosphate-buffered saline solution at 310 K, indicate 
that the bioactive components are released concurrently with bioMOF dissolution 
after ca. 30 min following a pseudo-first-order kinetic model. Furthermore, 
according to the semi-empirical Korsmeyer-Peppas kinetic model, this release is 
governed by a case-II mechanism, suggesting that the molecular transport is 
influenced by the relaxation of the NCC matrix. Preliminary in vitro results 
denote that the initial high concentration of glycerol in the NCC scaffold can 
be toxic in direct contact with human osteoblasts (HObs). However, when the 
excess of glycerol is diluted in the system (after the second day of the 
experiment), the direct and indirect assays confirm full biocompatibility and 
suitability for HOb proliferation.

DOI: 10.3390/gels10100631
PMCID: PMC11507435
PMID: 39451284

Conflict of interest statement: The authors declare no conflicts of interest.


3. Sci Rep. 2024 Oct 24;14(1):25251. doi: 10.1038/s41598-024-76200-8.

In vitro and computational investigation of antioxidant and anticancer 
properties of Streptomyces coeruleofuscus SCJ extract on MDA-MB-468 
triple-negative breast cancer cells.

Rammali S(1), Idir A(2)(3), Aherkou M(4)(5)(6), Ciobică A(7)(8)(9), Kamal 
FZ(10)(11), Aalaoui ME(12), Rahim A(13), Khattabi A(14), Abdelmajid Z(2), Aasfar 
A(15), Burlui V(16), Calin G(16), Mavroudis I(17), Bencharki B(14).

Author information:
(1)Laboratory of Agro-Alimentary and Health, Faculty of Sciences and Techniques, 
Hassan First University of Settat, B.P. 539, Settat, 26000, Morocco. 
rammali_fst@hotmail.fr.
(2)Agro-Industrial and Medical Biotechnology Laboratory, Team of Experimental 
Oncology and Natural Substances, Faculty of Sciences and Technology, Sultan 
Moulay Slimane University, Beni-Mellal, Morocco.
(3)Science and Technology Team, Higher School of Education and Training, Chouaîb 
Doukkali University, El Jadida, Morocco.
(4)Mohammed VI University of Sciences and Health, Casablanca, Morocco.
(5)Mohammed VI Centre for Research and Innovation (CM6RI), Casablanca, Morocco.
(6)Biotechnology Laboratory (MedBiotech), Bioinova Research Center, Faculty of 
Medicine and Pharmacy, Mohammed V University, Rabat, Morocco.
(7)Department of Biology, Faculty of Biology, Alexandru Ioan Cuza University of 
Iasi, 20th Carol I Avenue, Iasi, 700506, Romania. alin.ciobica@uaic.ro.
(8)Center of Biomedical Research, Iasi Branch, Romanian Academy, Teodor Codrescu 
2, Iasi, 700481, Romania. alin.ciobica@uaic.ro.
(9)Academy of Romanian Scientists, 3 Ilfov, Bucharest, 050044, Romania. 
alin.ciobica@uaic.ro.
(10)Higher Institute of Nursing Professions and Health Technical (ISPITS), 
Marrakech, 40000, Morocco.
(11)Laboratory of Physical Chemistry of Processes and Materials, Faculty of 
Sciences and Techniques, Hassan First University, Settat, 26000, Morocco.
(12)Regional Center of Agronomic Research of Settat, Tertiary Road 1406, At 5 Km 
from Settat, Settat, 26400, Morocco.
(13)Laboratory of Biochemistry, Neurosciences, Natural Ressources and 
Environment, Faculty of Sciences and Techniques, Hassan First University of 
Settat, B.P. 539, Settat, 26000, Morocco.
(14)Laboratory of Agro-Alimentary and Health, Faculty of Sciences and 
Techniques, Hassan First University of Settat, B.P. 539, Settat, 26000, Morocco.
(15)Plant and Microbial Biotechnology center, Moroccan Foundation for Advanced 
Science, Innovation and Research (MAScIR), Mohammed VI Polytechnic University, 
Ben Guerir, Morocco.
(16)"Ioan Haulica Institute", Apollonia University, Păcurari Street 11, Iasi, 
700511, Romania.
(17)Leeds Teaching Hospitals, NHS Trust, Leeds, LS97TF, UK.

This study aimed to explore the antioxidant potential of the ethyl acetate 
extract of Streptomyces coeruleofuscus SCJ strain, along with its inhibitory 
effects on the triple-negative human breast carcinoma cell line (MDA-MB-468). 
The ethyl acetate extract's total phenolic and flavonoid contents were 
quantified, and its antioxidant activity was investigated using DPPH 
(1,1-Diphenyl-2-picrylhydrazyl), ABTS (2,2'-azino-bis 
(3-ethylbenzothiazoline-6-sulphonic acid), and FRAP (Ferric Reducing Antioxidant 
Power) assays. Furthermore, the cytotoxic effect of the organic extract from 
Streptomyces coeruleofuscus SCJ on MDA-MB-468 cancer cells was assessed via the 
crystal violet assay. In tandem, a thorough computational investigation was 
conducted to explore the pharmacokinetic properties of the identified components 
of the extract, utilizing the SwissADME and pKCSM web servers. Additionally, the 
molecular interactions between these components and Estrogen Receptor Beta, 
identified as a potential target, were probed through molecular docking studies. 
The results revealed that ethyl acetate extract of SCJ strain exhibited 
remarkable antioxidant activity, with 39.899 ± 1.56% and 35.798 ± 0.082% 
scavenging activities against DPPH and ABTS, respectively, at 1 mg/mL. The 
extract also displayed significant ferric reducing power, with a concentration 
of 1.087 ± 0.026 mg ascorbic acid equivalents per mg of dry extract. 
Furthermore, a strong positive correlation (p < 0.0001) between the antioxidant 
activity, the polyphenol and the flavonoid contents. Regarding anticancer 
activity, the SCJ strain extract demonstrated significant anticancer activity 
against TNBC MDA-MB-468 cancer cells, with an inhibition percentage of 
62.76 ± 0.62%, 62.67 ± 0.93%, and 58.07 ± 4.82% at 25, 50, and 100 µg/mL of the 
extract, respectively. The HPLC-UV/vis analysis revealed nine phenolic 
compounds: gallic acid, sinapic acid, p-coumaric acid, cinnamic acid, 
trans-fereulic acid, syringic acid, chloroqenic acid, ellagic acid, epicatechin. 
Streptomyces coeruleofuscus SCJ showed promise for drug discovery, exhibiting 
antioxidant and anticancer effects.

© 2024. The Author(s).

DOI: 10.1038/s41598-024-76200-8
PMCID: PMC11502701
PMID: 39448707 [Indexed for MEDLINE]

Conflict of interest statement: The authors declare no competing interests.


4. Biochem Biophys Res Commun. 2024 Nov 19;734:150777. doi: 
10.1016/j.bbrc.2024.150777. Epub 2024 Oct 1.

Syringic acid improves cyclophosphamide-induced immunosuppression in a mouse 
model.

Mughal KS(1), Ikram M(2), Uddin Z(1), Rashid A(1), Rashid U(3), Khan M(1), Zehra 
N(1), Mughal US(4), Shah N(1), Amirzada I(1).

Author information:
(1)Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, 
Abbottabad, 22060, Khyber Pakhtunkhwa, Pakistan.
(2)Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, 
Abbottabad, 22060, Khyber Pakhtunkhwa, Pakistan. Electronic address: 
ikram@cuiatd.edu.pk.
(3)Department of Chemistry, COMSATS University Islamabad, Abbottabad Campus, 
Abbottabad, 22060, Khyber Pakhtunkhwa, Pakistan.
(4)Department of Medicine, Ayub Teaching Hospital, Abbottabad, 22040, Khyber 
Pakhtunkhwa, Pakistan.

Syringic acid (SA), a naturally occurring phenolic substance present in many 
edible plants and fruits, has been shown to have potential in immunoenhancement 
applications. In this study, we investigated the immunomodulatory effects of SA 
in mitigating cyclophosphamide (CYP)-induced immunosuppression in BALB/c mice 
using doxycycline as a positive control. SA administration prevented immune 
organ atrophy and morphological changes in the thymus, spleen, and bone marrow 
induced by CYP treatment in mice while also showing a dose-dependent enhancement 
of thymus and spleen indices compared to mice treated with CYP alone. 
Furthermore, SA improved thymocyte and splenocyte proliferation and exhibited 
significant antioxidant activity by reducing the elevated levels of 
malondialdehyde induced by CYP treatment. SA treatment effectively restored 
white blood cell (WBC) and lymphocyte counts to normal levels in CYP-treated 
animals, and the protective effects of CYP on immunological tissues were 
confirmed through histopathological examination. Moreover, SA treatment 
upregulated the expression of IL-6, IL-7, IL-15, and FoxN1. Finally, molecular 
docking studies revealed that binding energy values predicted minor inhibition 
potential toward IL-6, IL-7, FoxN1, IL-15, STAT3, STAT5, and JAK3. Overall, our 
findings suggest that SA treatment has the potential to reduce CYP-induced 
immunosuppression and may have applications as an immunologic adjuvant or 
functional food additive in chemotherapy.

Copyright © 2024 Elsevier Inc. All rights reserved.

DOI: 10.1016/j.bbrc.2024.150777
PMID: 39383831 [Indexed for MEDLINE]

Conflict of interest statement: Declaration of competing interest The authors 
declare no conflict of interest.


5. PLoS One. 2024 Oct 9;19(10):e0311802. doi: 10.1371/journal.pone.0311802. 
eCollection 2024.

Active packaging coating based on Lepidium sativum seed mucilage and propolis 
extract: Preparation, characterization, application and modeling the 
preservation of buffalo meat.

Majdi F(1), Alizadeh Behbahani B(1), Barzegar H(1), Mehrnia MA(1), Taki M(2).

Author information:
(1)Faculty of Animal Science and Food Technology, Department of Food Science and 
Technology, Agricultural Sciences and Natural Resources University of Khuzestan, 
Mollasani, Iran.
(2)Faculty of Agricultural Engineering and Rural Development, Department of 
Agricultural Machinery and Mechanization Engineering, Agricultural Sciences and 
Natural Resources University of Khuzestan, Mollasani, Iran.

Buffalo meat is naturally perishable, making it susceptible to spoilage due to 
its high moisture content and vulnerability to microbial contamination. Edible 
coatings have attracted attention as a packaging method that can prolong the 
shelf life of meat. The study aimed to examine the impact of a combination of 
Lepidium sativum mucilage (LS) coating and propolis extract (PE) on prolonging 
the shelf life of buffalo meat. The chemical characteristics (chemical 
compounds, total phenol content (TPC), total flavonoid content (TFC), 
antioxidant activity, and cytotoxicity) and antimicrobial activity of the PE 
(disk diffusion agar, well diffusion agar, minimum inhibitory concentration, and 
minimum bactericidal concentration) were investigated. The effect of the PE on 
the cell wall of pathogenic bacteria was examined using a scanning electron 
microscope. Biological properties of LS (TPC, TFC, antioxidant activity and 
antimicrobial effect (pour plate method)) was investigated. Different 
concentrations of PE (0, 0.5, 1.5, and 2.5%) were added to the coating mixture 
containing LS, and their effects on extending the shelf life of buffalo meat 
samples stored at 4°C for 9 days were assessed. The PE included gallic acid, 
benzoic acid, syringic acid, 4-3 dimethoxy cinnamic acid, p-coumaric acid, 
myricetin, caffeic acid, luteolin, chlorogenic acid, and apigenin. The PE was 
determined to have a TPC of 36.67 ± 0.57 mg GAE/g and a TFC of 48.02 ± 0.65 mg 
QE/g. The extract's radical scavenging activity ranged from 0 to 76.22% for DPPH 
radicals and from 0 to 50.31% for ABTS radicals. The viability of C115 HeLa cell 
was observed to be 94.14 μg/mL. The PE and LS, exhibited strong antimicrobial 
properties against pathogenic bacteria. The LS was determined to have a TPC of 
15.23 ± 0.43 mg GAE/g and a TFC of 11.51± 0.61 mg QE/g. The LS was determined to 
have a DPPH of 429.65 ± 1.28 μg/mL and a ABTS of 403.59 ± 1.46 μg/mL. The 
microbiological analysis revealed that the LS+2.5%PE treatment was the most 
effective in inhibiting the growth of total viable count (6.23 vs. 8.00 log 
CFU/g), psychrotrophic bacteria count (3.71 vs. 4.73 log CFU/g), coliforms count 
(2.78 vs. 3.70 log CFU/g), and fungi count (2.39 vs. 3.93 log CFU/g) compared to 
the control sample. The addition of PE to the edible coating also demonstrated a 
concentration-dependent effect on preserving the moisture, pH, color, and 
hardness of the buffalo meat. Sensory evaluation results suggested that 
incorporating PE into the edible coating extended the shelf life of buffalo meat 
by three days. In the second stage of this paper, this investigation employed 
two distinct forecasting methodologies: the Radial Basis Function (RBF) and the 
Support Vector Machine (SVM), to predict a range of quality indicators for 
coated meat products. Upon comparison, the RBF model exhibited a higher level of 
accuracy, showcasing its exceptional capacity to closely match the experimental 
outcomes. Therefore, this type of food coating, renowned for its strong 
antimicrobial properties, has the potential to effectively package and preserve 
perishable and delicate food items, such as meat.

Copyright: © 2024 Majdi et al. This is an open access article distributed under 
the terms of the Creative Commons Attribution License, which permits 
unrestricted use, distribution, and reproduction in any medium, provided the 
original author and source are credited.

DOI: 10.1371/journal.pone.0311802
PMCID: PMC11463836
PMID: 39383129 [Indexed for MEDLINE]

Conflict of interest statement: The authors have declared that no competing 
interests exist.