Psychoactive Plant Database - Neuroactive Phytochemical Collection

1. Anim Cells Syst (Seoul). 2024 Nov 1;28(1):536-550. doi: 
10.1080/19768354.2024.2417458. eCollection 2024.

AP001885.4 promotes the proliferation of esophageal squamous cell carcinoma 
cells by histone lactylation- and NF-κB (p65)-dependent transcription activation 
and METTL3-mediated mRNA stability of c-myc.

Fu C(1), Jiang W(2), Wang C(1), Song SJ(1), Tao H(1), Zhang XG(1), Li WT(1), Jin 
X(1), Yu BB(3), Hao JJ(4), Sun WJ(5), Bai J(1), Shi ZZ(1).

Author information:
(1)Medical School, Kunming University of Science and Technology, Kunming, 
People's Republic of China.
(2)Department of Thoracic Surgery, the Affiliated Hospital of Kunming University 
of Science and Technology and First People's Hospital of Yunnan Province, 
Kunming, People's Republic of China.
(3)Department of Anus & Intestine Surgery, Kunming Municipal Hospital of 
Traditional Chinese Medicine, Kunming, People's Republic of China.
(4)State Key Laboratory of Molecular Oncology, Center for Cancer Precision 
Medicine, National Clinical Research Center for Cancer/Cancer Hospital, National 
Cancer Center, Chinese Academy of Medical Sciences (CAMS), Peking Union Medical 
College (PUMC), Beijing, People's Republic of China.
(5)Nephrology Division, Pu'er People's Hospital, Pu'er, People's Republic of 
China.

Esophageal squamous cell carcinoma (ESCC) is an aggressive malignant neoplasm, 
and up to now, the role of long non-coding RNA (lncRNA) AP001885.4 in cancer, 
including ESCC, is absolutely unclear. The GEPIA database was applied to 
identify differentially expressed and prognosis-associated genes in esophageal 
cancer (ESCA). CCK-8, colony formation, Western blot, and qRT-PCR methods were 
harnessed to investigate the role and mechanism of AP001885.4 in esophageal 
carcinogenesis. By analyzing TCGA data in the GEPIA database, two lncRNAs were 
selected. AP001885.4 was overexpressed and positively associated with the 
unfavorable outcome of ESCC patients, and LINC001786 was under-expressed and 
negatively linked with the poor prognosis. Knockdown of AP001885.4 suppressed 
the proliferation and colony formation of ESCC cells. Importantly, the silence 
of AP001885.4 downregulated c-myc. Mechanically, the knockdown of AP001885.4 
reduced METTL3 expression and m6A modification in c-myc mRNA, and METTL3 
positively regulated c-myc. Furthermore, the knockdown of AP001885.4 diminished 
histone lactylation and NF-κB (p65) expression, and the protein lactylation 
inhibitors (2-DG, 2-deoxy-D-glucose and oxamate) and the NF-κB inhibitor 
(JSH-23) also lessened c-myc expression. Consequently, our findings suggested 
that AP001885.4 promoted the proliferation of esophageal squamous cell carcinoma 
cells by histone lactylation- and NF-κB (p65)-dependent transcription activation 
and METTL3-mediated mRNA stability of c-myc.

© 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & 
Francis Group.

DOI: 10.1080/19768354.2024.2417458
PMCID: PMC11536669
PMID: 39502790

Conflict of interest statement: No potential conflict of interest was reported 
by the author(s).


2. Sci Rep. 2024 Oct 30;14(1):26081. doi: 10.1038/s41598-024-76548-x.

Structural dynamics of human deoxyuridine 5'-triphosphate nucleotidohydrolase 
(dUTPase).

Sarre R(1), Dobrovolska O(1), Lundström P(2), Turcu D(3), Agback T(4), Halskau 
Ø(3), Isaksson J(5)(6).

Author information:
(1)Department of Chemistry, UiT the Arctic University of Norway, PO Box 6050, 
Stakkevollan, 9037, Langnes, Tromsø, Norway.
(2)Department of Physics, Chemistry and Biology, Linköping University, 581 83, 
Linköping, Sweden.
(3)Department of Biological Sciences, University of Bergen, Box 7803, 5020, 
Bergen, Norway.
(4)Department of Molecular Sciences, Swedish University of Agricultural 
Sciences, P.O Box 7070, 750 07, Uppsala, Sweden.
(5)Department of Chemistry, UiT the Arctic University of Norway, PO Box 6050, 
Stakkevollan, 9037, Langnes, Tromsø, Norway. johan.isaksson@uit.no.
(6)Department of Pharmacy, UiT the Arctic University of Norway, PO Box 6050, 
Stakkevollan, 9037, Langnes, Tromsø, Norway. johan.isaksson@uit.no.

Structural- and functional heterogeneity, as well as allosteric regulation, in 
homo-monomeric enzymes is a highly active area of research. One such enzyme is 
human nuclear-associated deoxyuridine 5'-triphosphate nucleotidohydrolase 
(dUTPase), which has emerged as an interesting drug target in combination 
therapy with traditional nucleotide analogue treatment of cancer. We report, for 
the first time, a full structural dynamics study of human dUTPase by NMR. 
dUTPase has been investigated in terms of structural dynamics in its apo form, 
in complex with the modified substrate resistant to hydrolysis, 2'-deoxyuridine 
5'-α,β-imido-triphosphate (dUpNHpp), as well as the product, 
2'-deoxy-uridine-monophosphate (dUMP). The apo form of the enzyme displayed slow 
dynamics in the milli- to microsecond regime in relaxation dispersion 
experiments, which was further slowed down to observable heterogeneity upon 
substrate-analogue binding. The results suggest that the non-hydrolysable 
substrate-analogue traps the enzyme in the conformational isomerization step 
that has been previously suggested to be part of the enzyme catalysis kinetics 
cycle. The observed heterogeneity fits well with the pattern expected to emerge 
from the suggested kinetic model, and no evidence for homotropic allosterism was 
found. The heatmaps of the slow dynamics, chemical shift perturbation upon 
substrate binding and conserved regions of the enzyme sequence all displayed a 
similar pattern, which suggests that the structural dynamics is finely tuned and 
important for the biological function of the enzyme for binding, conformational 
shift, catalysis and substrate release.

© 2024. The Author(s).

DOI: 10.1038/s41598-024-76548-x
PMCID: PMC11525568
PMID: 39477983 [Indexed for MEDLINE]

Conflict of interest statement: The authors declare no competing interests.


3. PET Clin. 2024 Oct 29:S1556-8598(24)00074-9. doi: 10.1016/j.cpet.2024.09.003. 
Online ahead of print.

Brain Tumor Assessment: Integrating PET/Computed Tomography and MR Imaging 
Modalities.

Steenhout C(1), Deprez L(1), Hustinx R(1), Withofs N(2).

Author information:
(1)Division of Nuclear Medicine and Oncological Imaging, University Hopsital of 
Liège, Avenue de l'Hôpital 1, Liège B-4000, Belgium.
(2)Division of Nuclear Medicine and Oncological Imaging, University Hopsital of 
Liège, Avenue de l'Hôpital 1, Liège B-4000, Belgium. Electronic address: 
nwithofs@chuliege.be.

While MR imaging is the main imaging modality to assess brain tumors, PET 
imaging has a specific role. Among the many tracers that have been proposed and 
are still being developed, 2-[18F]fluoro-2-deoxy-d-glucose ([18F]FDG) and 
O-(2-[18F]-fluoroethyl)-l-tyrosine ([18F]FET) PET remain the most solidly 
established in the clinics. In particular, [18F]FET has gained increased 
acceptance due to its higher sensitivity. In this paper, we present an overview 
of the current clinical status of brain tumor imaging, with emphasis on PET 
imaging.

Copyright © 2024 Elsevier Inc. All rights reserved.

DOI: 10.1016/j.cpet.2024.09.003
PMID: 39477722

Conflict of interest statement: Disclosure The authors received no financial 
support for the study, authorship, and/or publication of this article.


4. Alzheimers Res Ther. 2024 Oct 29;16(1):241. doi: 10.1186/s13195-024-01604-7.

Assessing the metabolism of the olfactory circuit by use of (18)F-FDG PET-CT 
imaging in patients suspected of suffering from Alzheimer's disease or 
frontotemporal dementia.

Loewenstein DSL(1), van Grinsven M(2), de Pont C(3), Dautzenberg PLJ(4), van 
Strien AM(4), Henssen D(2)(3).

Author information:
(1)Department of Medical Imaging, Radboud University Medical Center, Geert 
Grooteplein Zuid 10, Nijmegen, 6525 EZ, The Netherlands. 
daniel.loewenstein@radboudumc.nl.
(2)Department of Medical Imaging, Radboud University Medical Center, Geert 
Grooteplein Zuid 10, Nijmegen, 6525 EZ, The Netherlands.
(3)Department of Medical Imaging, Jeroen Bosch Hospital, 's Hertogenbosch, The 
Netherlands.
(4)Department of Geriatrics, Jeroen Bosch Hospital, 's Hertogenbosch, The 
Netherlands.

PURPOSE: The loss of olfactory function is known to occur in patients suffering 
from (behavioral variant) frontotemporal dementia ((bv)FTD) and Alzheimer's 
disease (AD), although different pathophysiological mechanisms underpin this 
clinical symptom in both disorders. This study assessed whether brain metabolism 
of the olfactory circuit as assessed by positron emission tomography (PET) 
imaging with 2-[fluorine-18]fluoro-2-deoxy-d-glucose ([18F]-FDG) can distinguish 
these entities in different subsets of patients.
METHODS: Patients presenting with cognitive decline were included from a 
prospectively kept database: (1) bvFTD patients, (2) AD patients and (3) 
patients with logopenic primary progressive aphasia (PPA). Metabolic rates were 
calculated for different regions of the olfactory circuit for each subgroup and 
compared with a cohort of subjects with normal brain metabolism. Additionally, 
in patients with a logopenic PPA pattern on PET-imaging, statistical parametric 
mapping (SPM) analysis was performed.
RESULTS: The metabolism of subdivisions of the olfactory circuit as assessed by 
[18F]-FDG PET brain imaging to bvFTD and AD from control subjects resulted in 
sensitivity/specificity rates of 95/87.5% and 80/83.3%, respectively. A 
sensitivity/specificity rate of 100/87.5% was achieved when used to 
differentiate AD from bvFTD. In patients with the PPA pattern on imaging, the 
underlying cause (either FTD or AD) could be determined with a 
sensitivity/specificity rate of 88/82%. SPM analysis concurred that different 
regions of the olfactory circuit were affected in patients suffering from AD PPA 
or bvFTD PPA.
CONCLUSION: Metabolic dysfunction in the olfactory circuit is different in 
various neurodegenerative disorders. Further investigation of the correlations 
between the cerebral metabolism and the mechanisms which drive olfactory 
dysfunction is needed.

© 2024. The Author(s).

DOI: 10.1186/s13195-024-01604-7
PMCID: PMC11520854
PMID: 39472983 [Indexed for MEDLINE]

Conflict of interest statement: The authors declare no competing interests.


5. Carbohydr Res. 2024 Oct 25;545:109300. doi: 10.1016/j.carres.2024.109300.
Online  ahead of print.

Effect of bacterial dissociation on lipopolysaccharide structure: A study of 
O-polysaccharide from the marine bacterium Pseudoalteromonas agarivorans KMM 232 
(O-form).

Kokoulin MS(1), Belova VS(2), Romanenko LA(3).

Author information:
(1)G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of 
the Russian Academy of Sciences, 159 Prospekt 100 Let Vladivostoku, Vladivostok, 
690022, Russia. Electronic address: maxchem@mail.ru.
(2)G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of 
the Russian Academy of Sciences, 159 Prospekt 100 Let Vladivostoku, Vladivostok, 
690022, Russia; Far Eastern Federal University, 10 Ajax Bay, Russky Island, 
Vladivostok, 690922, Russia.
(3)G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of 
the Russian Academy of Sciences, 159 Prospekt 100 Let Vladivostoku, Vladivostok, 
690022, Russia.

The lipopolysaccharide (LPS) was obtained from a bacterium Pseudoalteromonas 
agarivorans KMM 232 (O-form) isolated from a seawater sample collected at a 
depth of 500 m. The O-polysaccharide (OPS) was isolated by mild acid degradation 
of the LPS and studied by chemical methods along with 1D and 2D 1H and 13C NMR 
spectroscopy, including 1H,1H COSY, 1H,1H TOCSY, 1H,1H ROESY and 1H,13C HSQC, 
and 1H,13C HMBC experiments. The following new structure of the OPS from P. 
agarivorans KMM 232 (O-form) containing 2-acetamido-2-deoxy-d-glucose 
(D-GlcNAc), d-glucose (D-Glc), d-glucuronic acid (D-GlcA), 
4,6-O-[(R)-1-carboxyethylidene]-d-galactose [D-Galp4,6 (R-Pyr)] and two residues 
of d-galactose (D-Gal) was established.

Copyright © 2024 Elsevier Ltd. All rights reserved.

DOI: 10.1016/j.carres.2024.109300
PMID: 39471535

Conflict of interest statement: Declaration of competing interest