1. ACS Omega. 2024 Oct 20;9(43):43453-43468. doi: 10.1021/acsomega.4c04795.
eCollection 2024 Oct 29.
Urine Metabolomics during a Legume Diet Intervention Suggests Altered Metabolic
Signatures and Potential New Intake Markers: First Insights.
Ferreira H(1), Duarte D(2), Rodrigues JA(2), Vasconcelos MW(1), Pinto E(1)(3),
Gil AM(2).
Author information:
(1)CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola
Superior de Biotecnologia, Universidade Católica Portuguesa, Porto 4200-072,
Portugal.
(2)Department of Chemistry and CICECO-Aveiro Institute of Materials,
Universidade de Aveiro, Campus Universitário de Santiago, Aveiro 3810-193,
Portugal.
(3)EPIUnit - Instituto de Saúde Pública, Universidade do Porto, Rua das Taipas
135, Porto 4050-600, Portugal.
Given the general increase in legume consumption worldwide, there is a need to
characterize the resulting human metabolic adaptations in order to demonstrate
potential legume diet/health relationships. A nuclear magnetic resonance (NMR)
metabolomics urine study was carried out on a small cohort (n = 18) to
characterize the excretory effects of a pilot longitudinal 8-week legume-based
dietary intervention. Despite the expected high interindividual variability in
the excreted metabolome, the results suggested a nonlinear metabolic response,
with higher metabolic activity in the first 4 weeks and a tendency toward
baseline at the end of the intervention. The excretion of isoleucine, leucine,
and threonine increased, along with metabolite changes suggestive of activation
of the tricarboxylic acid cycle (through anaplerosis), ketogenesis, fat
catabolism, and glycoprotein biosynthesis. Gut microbiota adaptations were also
suggested based on the increased excretion of 2-hydroxyisobutyrate, allantoin,
and hippurate. Increased levels of trigonelline were consistent with its role as
a legume intake marker, whereas malonate and pseudouridine were suggested as
possible additional markers. Correlation of NMR data with nutritional parameters
aided putative explanatory hypotheses to be advanced. Our results suggest a
dynamic response to legume consumption, mainly through increased amino acid
excretion and altered energy metabolism, while advancing potential new markers
of legume intake. These results require confirmation in larger cohorts but pave
the way for an informed interpretation of the effects of legume-based diets on
human health.
© 2024 The Authors. Published by American Chemical Society.
DOI: 10.1021/acsomega.4c04795
PMCID: PMC11525520
PMID: 39494014
Conflict of interest statement: The authors declare no competing financial
interest.
2. Clin Nutr. 2024 Oct 12;43(12):146-157. doi: 10.1016/j.clnu.2024.10.009. Online
ahead of print.
Increasing plant protein in the diet induces changes in the plasma metabolome
that may be beneficial for metabolic health. A randomized crossover study in
males.
Lépine G(1), Mariotti F(2), Tremblay-Franco M(3), Courrent M(4), Verny MA(5),
David J(5), Mathé V(2), Jame P(6), Anchisi A(6), Lefranc-Millot C(7), Perreau
C(7), Guérin-Deremaux L(7), Chollet C(8), Castelli F(8), Chu-Van E(8), Huneau
JF(2), Rémond D(5), Pickering G(4), Fouillet H(9), Polakof S(10).
Author information:
(1)Université Clermont Auvergne, INRAE, UNH, 63000, Clermont-Ferrand, France;
Université Paris-Saclay, AgroParisTech, INRAE, UMR PNCA, 91120, Palaiseau,
France.
(2)Université Paris-Saclay, AgroParisTech, INRAE, UMR PNCA, 91120, Palaiseau,
France.
(3)Toxalim - Research Centre in Food Toxicology, Toulouse University, INRAE,
ENVT, INP-Purpan, UT3, F-31300, Toulouse, France; MetaboHUB-MetaToul, National
Infrastructure of Metabolomics and Fluxomics, Toulouse, 31077, France.
(4)CHU Clermont-Ferrand, Inserm CIC 1405, France.
(5)Université Clermont Auvergne, INRAE, UNH, 63000, Clermont-Ferrand, France.
(6)Universite Claude Bernard Lyon 1, CNRS, ISA, UMR5280, 5 rue de la Doua,
F-69100 Villeurbanne, France.
(7)Roquette Frères, Lestrem, France.
(8)CEA, INRAE, Département Médicaments et Technologies pour la Santé (DMTS),
MetaboHUB, Université Paris-Saclay, 91191 Gif-sur-Yvette, France.
(9)Université Paris-Saclay, AgroParisTech, INRAE, UMR PNCA, 91120, Palaiseau,
France. Electronic address: helene.fouillet@agroparistech.fr.
(10)Université Clermont Auvergne, INRAE, UNH, 63000, Clermont-Ferrand, France.
Electronic address: sergio.polakof@inrae.fr.
BACKGROUND & AIM: Dietary shifts replacing animal protein (AP) with plant
protein (PP) sources have been associated with lowering cardiometabolic risk
(CMR), but underlying mechanisms are poorly characterized. This nutritional
intervention aims to characterize the metabolic changes induced by diets
containing different proportions of AP and PP sources in males at CMR.
DESIGN: This study is a 4-week, crossover, randomized, controlled-feeding trial
in which 19 males with CMR followed two diets providing either 36 % for the
control diet (CON-D) or 64 % for the flexitarian diet (FLEX-D) of total protein
intake from PP sources. Plasma nontargeted metabolomes (LC-MS method) were
measured in the fasted state and after a high-fat challenge meal at the end of
each intervention arm. Lipogenesis and protein synthesis fluxes, flow-mediated
dilatation (FMD) and gluco-lipidic responses were assessed after the challenge
meal. Data were analyzed with mixed models, and univariate and multivariate
models for metabolomics data.
RESULTS: In both arms CMR improved with time, with decreased body weight
(-0.9 %), insulin resistant (-34 %, HOMA-IR, Homeostatic Model Assessment for
Insulin Resistance) and low-density lipoproteins (LDL)-cholesterol (-11 %). Diet
had no effect on FMD or metabolic fluxes, but a trend (0.05 1 and were predictive of DN. When
conducting multivariate ROC analysis with a combination of clinical factors and
urinary metabolites, the area under the curve was 1.000.
CONCLUSIONS: The combination of clinical factors and urinary metabolites is
highly valuable for predicting biopsy-confirmed DN in patients with T2DM.
Copyright © 2023 Elsevier B.V. All rights reserved.
DOI: 10.1016/j.diabres.2023.110986
PMID: 39445434 [Indexed for MEDLINE]
Conflict of interest statement: Declaration of Competing Interest The authors
declare that they have no known competing financial interests or personal
relationships that could have appeared to influence the work reported in this
paper.
5. Plant Cell Rep. 2024 Oct 16;43(11):264. doi: 10.1007/s00299-024-03356-y.
Cellulose synthase-like OsCSLD4: a key regulator of agronomic traits, disease
resistance, and metabolic indices in rice.
Zhang G(1)(2)(3), Yang Z(1)(2), Zhou S(1)(2), Zhu J(1)(2), Liu X(1)(2), Luo
J(4)(5).
Author information:
(1)School of Tropical Agriculture and Forestry, Hainan University, Haikou,
570228, China.
(2)School of Breeding and Multiplication, Hainan University, Sanya, 572025,
China.
(3)Yazhou Bay Seed Laboratory, Sanya, 572025, China.
(4)School of Breeding and Multiplication, Hainan University, Sanya, 572025,
China. jie.luo@hainanu.edu.cn.
(5)Yazhou Bay National Laboratory, Sanya, 572025, China. jie.luo@hainanu.edu.cn.
Cellulose synthase-like OsCSLD4 plays a pivotal role in regulating diverse
agronomic traits, enhancing resistance against bacterial leaf blight, and
modulating metabolite indices based on the multi-omics analysis in rice. To
delve deeper into this complex network between agronomic traits and metabolites
in rice, we have compiled a dataset encompassing genome, phenome, and
metabolome, including 524 diverse accessions, 11 agronomic traits, and 841
metabolites, enabling us to pinpoint eight hotspots through GWAS. We later
discovered four distinct metabolite categories, encompassing 15 metabolites that
are concurrently present on the QTL qC12.1, associated with leaf angle of flag
and spikelet length, and finally focused the cellulose synthase-like OsCSLD4,
which was pinpointed through a rigorous process encompassing sequence variation,
haplotype, ATAC, and differential expression across diverse tissues. Compared to
the wild type, csld4 exhibited significant reductions in the plant height, flag
leaf length, leaf width, spikelet length, 1000-grain weight, grain width, grain
thickness, fertility, yield per plant, and bacterial blight resistance. However,
there were significant increase in tiller numbers, degree of leaf rolling,
flowering period, growth period, grain length, and empty kernel rate.
Furthermore, the content of four polyphenol metabolites, excluding metabolite
N-feruloyltyramine (mr1268), notably rose, whereas the levels of the other three
polyphenol metabolites, smiglaside C (mr1498), 4-coumaric acid (mr1622), and
smiglaside A (mr1925) decreased significantly in mutant csld4. The content of
amino acid L-tyramine (mr1446) exhibited a notable increase, whereas the
alkaloid trigonelline (mr1188) displayed a substantial decrease among the
mutants. This study offered a comprehensive multi-omics perspective to analyze
the genetic mechanism of OsCSLD4, and breeders can potentially enhance rice's
yield, bacterial leaf blight resistance, and metabolite content, leading to more
sustainable and profitable rice production.
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany,
part of Springer Nature.
DOI: 10.1007/s00299-024-03356-y
PMID: 39414689 [Indexed for MEDLINE]