Psychoactive Plant Database - Neuroactive Phytochemical Collection

1. Food Chem. 2024 Oct 30;464(Pt 3):141832. doi: 10.1016/j.foodchem.2024.141832. 
Online ahead of print.

Sensory qualities markers of n-3 PUFA enriched fresh pork meat fattened by 
linseed oil and selenium methionine.

Hui T(1), Li Q(2), Fang Z(3), Li R(4), Sun Y(4), Li J(2), Yang Y(4).

Author information:
(1)Key Laboratory of Agricultural Product Processing and Nutrition Health 
(Co-construction by Ministry and Province), Ministry of Agriculture and Rural 
Affairs, China, Ya'an 625014, China; College of Food Science, Sichuan 
Agricultural University, Ya'an 625014, China. Electronic address: 
huiteng@sicau.edu.cn.
(2)Institute of Food Science and Technology, Chinese Academy of Agricultural 
Sciences, Beijing 100193, PR China.
(3)Key Laboratory of Agricultural Product Processing and Nutrition Health 
(Co-construction by Ministry and Province), Ministry of Agriculture and Rural 
Affairs, China, Ya'an 625014, China; College of Food Science, Sichuan 
Agricultural University, Ya'an 625014, China. Electronic address: 
zfang@sicau.edu.cn.
(4)Key Laboratory of Agricultural Product Processing and Nutrition Health 
(Co-construction by Ministry and Province), Ministry of Agriculture and Rural 
Affairs, China, Ya'an 625014, China; College of Food Science, Sichuan 
Agricultural University, Ya'an 625014, China.

This study revealed the differences of sensory markers between traditional pork 
meat, fatten by 3 % soybean oil, and n-3 PUFA enriched pork meat, which was 
fattened through a pig diet supplemented with 3 % linseed oil and a combination 
of 3 % linseed oil with 0.3 mg/kg of selenium methionine. E-nose (tongue) 
analysis indicated pronounced differences in odor and taste profiles of pork 
meats between control group and linseed oil treatment group. 1-octen-3-ol and 
5'-IMP were identified as the volatile and non-volatile markers of n-3 PUFA 
enriched pork meat, respectively. The volatile marker 1-octen-3-ol was 
decreased, while the non-volatile marker of 5'-IMP was increased in n-3 PUFA 
enriched pork meat fattened by linseed oil, as compared to pork fattened by 
soybean oil. The supplement of 0.3 mg/kg of selenium methionine to 3 % linseed 
oil diet had no significant effect on 1-octen-3-ol, but mitigated the increase 
of 5'-IMP and enhanced the meat's cohesiveness.

Copyright © 2024 Elsevier Ltd. All rights reserved.

DOI: 10.1016/j.foodchem.2024.141832
PMID: 39504892

Conflict of interest statement: Declaration of competing interest The authors 
declare that they have no known competing financial interests or personal 
relationships that could have appeared to influence the work reported in this 
paper.


2. Biotechnol Appl Biochem. 2024 Nov 4. doi: 10.1002/bab.2688. Online ahead of 
print.

Virtual screening of plant-derived molecules against zinc-dependent imipenemases 
in class B metallo-β-lactamases of Acinetobacter baumannii.

Gopikrishnan M(1), Doss C GP(1).

Author information:
(1)Department of Integrative Biology, School of Biosciences and Technology, 
Vellore Institute of Technology, Vellore, India.

Metallo-β-lactamases (MBLs), enzymes of class B, employ zinc ions to degrade 
β-lactam antibiotics such as penicillins, cephalosporins, carbapenems, and 
cephamycins. Carbapenem-resistant Acinetobacter baumannii (CRAB) is linked to 
the existence of carbapenemase enzymes such as oxacillinase and MBL. The most 
prevalent resistance mechanisms include imipenemases (IMP), verona 
integron-encoded MBL, and New Delhi MBL-1. The effectiveness of current 
antibiotics against the MBL enzyme is limited due to the presence of metal ions, 
underscoring the need for new antimicrobial agents. Recent research has 
demonstrated that natural compounds can effectively inhibit MBL. This study aims 
to screen natural phytochemicals against IMP-2 MBL using in silico virtual 
screening techniques via AutoDock Vina and molecular dynamic simulations with 
GROMACS for 200 ns, followed by molecular mechanics/Poisson‒Boltzmann surface 
area analysis. This procedure identified new lead molecules against A. baumannii 
that produce IMP. A total of 588 natural compounds were screened against IMP, 
along with the imipenem substrate and known inhibitors of L-captopril. The top 
four compounds, N025-0038 (NC1), N062-0008 (NC2), eupalitin, and Rosmorinic 
acid, demonstrated binding affinities of ‒8.5, ‒8.4, ‒7.5, and ‒7.2 kcal/mol, 
respectively. The structural stability of these complexes was observed to be 
maintained throughout the simulation in a dynamic environment, as determined by 
molecular dynamics trajectory analysis, and all these compounds met the 
SWISS-ADME (adsorption, distribution, metabolism, and excretion) properties. NC1 
and NC2 compounds are considered potential drug molecules against IMP. However, 
while these selected compounds showed superior binding energy in computational 
analysis, further in vitro analysis is required to establish an effective drug 
regimen against A. baumannii that produces IMP.

© 2024 International Union of Biochemistry and Molecular Biology, Inc.

DOI: 10.1002/bab.2688
PMID: 39497263


3. Sci Rep. 2024 Nov 5;14(1):26723. doi: 10.1038/s41598-024-78205-9.

The characterization of an IncN-IncR fusion plasmid co-harboring bla(TEM-40), 
bla(KPC-2), and bla(IMP-4) derived from ST1393 Klebsiella pneumoniae.

Fang L(#)(1), Shen Y(#)(1), Chen R(1), Li C(1), Liu R(2), Jia Y(1), Qi S(1), Guo 
X(3).

Author information:
(1)Department of Laboratory Medicine, The First Affiliated Hospital of Zhengzhou 
University, Zhengzhou, 450052, China.
(2)Collaborative Innovation Center for Diagnosis and Treatment of Infectious 
Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious 
Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, 
Hangzhou, 310003, China.
(3)Department of Laboratory Medicine, The First Affiliated Hospital of Zhengzhou 
University, Zhengzhou, 450052, China. gxbing928@zzu.edu.cn.
(#)Contributed equally

Plasmids, as important genetic elements apart from chromosomes, often carry 
multiple resistance genes and various mobile genetic elements, enabling them to 
acquire more exogenous genes and confer additional resistance phenotypes to 
bacteria. Various carbapenem resistance genes are often located on IncN 
plasmids, and several reports have linked fusion plasmids to IncN plasmids. 
Therefore, this study aims to explore the emergence, molecular structure 
characteristics, and resistance features mediated by IncN fusion plasmids 
carrying multiple carbapenem resistance genes. In this study, species 
identification was performed using matrix-assisted laser desorption/ionization 
time-of-flight mass spectrometry (MALDI-TOF/MS). Polymerase chain reaction (PCR) 
was employed to detect the presence of carbapenem resistance genes in the 
strains. PCR-based replicon typing (PBRT) was used to identify IncN plasmids. 
Plasmids were analyzed through S1-nuclease pulsed-field gel electrophoresis 
(S1-PFGE), Southern blotting, conjugation experiments, and stability tests. 
Whole-genome sequencing (WGS) and antimicrobial susceptibility testing (AST) 
were conducted to characterize the target strains. Four strains containing IncN 
plasmids were identified: two Klebsiella pneumoniae, one Escherichia coli, and 
one Enterobacter cloacae, all harboring carbapenem resistance genes. Among them, 
two IncN plasmids (pFAHZZU7605-KPC-IMP and pFAHZZU7865-IMP) contained blaIMP-4 
and exhibited similar molecular structure characteristics. Notably, the 
pFAHZZU7605-KPC-IMP plasmid harbored both IncN and IncR replicons. We 
hypothesize that the pFAHZZU7605-KPC-IMP fusion plasmid resulted from the 
recombination of a pFAHZZU7865-IMP-like plasmid and an IncR-like plasmid. 
Further analysis of the plasmid's genetic elements revealed that insertion 
sequences ISKpn19 and ISKpn27 played crucial roles in the plasmid recombination 
and fusion process. In clinical settings, plasmids carrying different resistance 
genes can undergo fusion, mediated by genetic elements, thereby expanding the 
resistance spectrum of host bacteria. Hence, it is essential to enhance the 
monitoring and research of transposable elements to control the spread of 
multidrug-resistant bacteria.

© 2024. The Author(s).

DOI: 10.1038/s41598-024-78205-9
PMCID: PMC11535437
PMID: 39496788 [Indexed for MEDLINE]

Conflict of interest statement: The authors declare no competing interests.


4. NPJ Biofilms Microbiomes. 2024 Nov 4;10(1):118. doi:
10.1038/s41522-024-00592-8.

Kinetics of imidazole propionate from orally delivered histidine in mice and 
humans.

Warmbrunn MV(#)(1)(2), Attaye I(#)(3)(4)(5), Horak A(4), Banerjee R(4), Massey 
WJ(4), Varadharajan V(4), Rampanelli E(3), Hao Y(3), Dutta S(4), Nemet I(5), 
Aron-Wisnewsky J(6)(7), Clément K(6)(7), Koopen A(3), Wortelboer K(3)(8), Bergh 
PO(9), Davids M(3), Mohamed N(3), Kemper EM(10), Hazen S(4), Groen AK(3), van 
Raalte DH(3)(11), Herrema H(3), Backhed F(9)(12), Brown JM(4), Nieuwdorp M(3).

Author information:
(1)Department of Internal and Vascular Medicine, Amsterdam University Medical 
Centers, Location AMC, Amsterdam, The Netherlands. 
m.v.warmbrunn@amsterdamumc.nl.
(2)Amsterdam UMC, University of Amsterdam, Gastroenterology and Hepatology, 
Amsterdam Gastroenterology Endocrinology Metabolism, Amsterdam, Netherlands. 
m.v.warmbrunn@amsterdamumc.nl.
(3)Department of Internal and Vascular Medicine, Amsterdam University Medical 
Centers, Location AMC, Amsterdam, The Netherlands.
(4)Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, 
Cleveland, OH, USA.
(5)Department of Cardiovascular and Metabolic Sciences, Lerner Research 
Institute, Cleveland Clinic, Cleveland, OH, USA.
(6)Sorbonne Université, INSERM, Nutrition and Obesities; Systemic Approaches 
(NutriOmics), Sorbonne Université, Paris, France.
(7)Assistance Publique Hôpitaux de Paris,Pitie-Salpêtrière Hospital, Nutrition 
department, CRNH Ile de France, Paris, France.
(8)Amsterdam UMC, University of Amsterdam, Gastroenterology and Hepatology, 
Amsterdam Gastroenterology Endocrinology Metabolism, Amsterdam, Netherlands.
(9)Wallenberg Laboratory and Department of Molecular and Clinical Medicine, 
Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, 
Gothenburg, Sweden.
(10)Department of Clinical Pharmacology, Amsterdam University Medical Centers, 
Location AMC, Amsterdam, the Netherlands.
(11)VU University, Amsterdam Cardiovascular Sciences, Amsterdam, The 
Netherlands.
(12)Region Västra Götaland, Sahlgrenska University Hospital, Department of 
Clinical Physiology, Gothenburg, Sweden.
(#)Contributed equally

Imidazole Propionate (ImP), a gut-derived metabolite from histidine, affects 
insulin signaling in mice and is elevated in type 2 diabetes (T2D). However, the 
source of histidine and the role of the gut microbiota remain unclear. We 
conducted an intervention study in mice and humans, comparing ImP kinetics in 
mice on a high-fat diet with varying histidine levels and antibiotics, and 
assessed ImP levels in healthy and T2D subjects with histidine supplementation. 
Results show that dietary histidine is metabolized to ImP, with 
antibiotic-induced gut microbiota suppression reducing ImP levels in mice. In 
contrast, oral histidine supplementation resulted in increases in circulating 
ImP levels in humans, whereas antibiotic treatment increased ImP levels, which 
was associated with a bloom of several bacterial genera that have been 
associated with ImP production, such as Lactobacilli. Our findings highlight the 
gut microbiota's crucial role in regulating ImP and the complexity of 
translating mouse models to humans.

© 2024. The Author(s).

DOI: 10.1038/s41522-024-00592-8
PMCID: PMC11535228
PMID: 39496629 [Indexed for MEDLINE]

Conflict of interest statement: M.N. is a member of the scientific advisory 
board of Caelus Health and all honoraria are paid to the employer Amsterdam 
University Medical Centres. F.B. receives research funding from Biogaia AB, is 
co-founder and shareholder of Roxbiosens Inc and Implexion AB, and is on the 
scientific advisory board of Bactolife A/S. K.C. has held a collaborative 
research contract with Danone Research in the context of the MetaCardis project. 
D.H.v.R. has participated in advisory boards for AstraZeneca, Boehringer 
Ingelheim‐Eli Lilly Alliance, MSD, Novo Nordisk and Sanofi, and has received 
research grants from AstraZeneca, Boehringer Ingelheim‐Eli Lilly Alliance, MSD 
and Sanofi. All honoraria are paid to the employer of Amsterdam University 
Medical Centres. All other authors declare to have no related conflict of 
interest.


5. Carbohydr Polym. 2025 Jan 1;347:122670. doi: 10.1016/j.carbpol.2024.122670.
Epub  2024 Aug 28.

Chitosan based hydrogel for iron (III) chelation in biological conditions.

Salazar Ariza JF(1), Lux F(2), Da Cruz-Boisson F(3), Resende de Azevedo J(4), 
Vera R(5), Tillement O(6), Montembault A(3), David L(7).

Author information:
(1)Universite Claude Bernard Lyon 1, CNRS, Institut Lumière-Matière (ILM), UMR 
5306, 2 rue Victor Grignard, F-69622 Villeurbanne Cedex, France; Universite 
Claude Bernard Lyon 1, INSA de Lyon, Universite Jean Monnet, CNRS, Ingénierie 
des Matériaux Polymères (IMP), UMR 5223, 15 bd Latarjet, F-69622 Villeurbanne, 
France.
(2)Universite Claude Bernard Lyon 1, CNRS, Institut Lumière-Matière (ILM), UMR 
5306, 2 rue Victor Grignard, F-69622 Villeurbanne Cedex, France; Institut 
Universitaire de France (IUF), 75231 Paris, France.
(3)Universite Claude Bernard Lyon 1, INSA de Lyon, Universite Jean Monnet, CNRS, 
Ingénierie des Matériaux Polymères (IMP), UMR 5223, 15 bd Latarjet, F-69622 
Villeurbanne, France.
(4)Universite Claude Bernard Lyon 1, CNRS, Laboratoire d'Automatique, de Génie 
des Procédés et de Génie Pharmaceutique (LAGEPP), UMR5007, 3, rue Victor 
Grignard, Bâtiment CPE, F-69100 Villeurbanne Cedex, France.
(5)Universite Claude Bernard Lyon 1, Centre de Diffractométrie Henri 
Longchambon, 5 rue de La Doua, F-69100 Villeurbanne, France.
(6)Universite Claude Bernard Lyon 1, CNRS, Institut Lumière-Matière (ILM), UMR 
5306, 2 rue Victor Grignard, F-69622 Villeurbanne Cedex, France.
(7)Universite Claude Bernard Lyon 1, INSA de Lyon, Universite Jean Monnet, CNRS, 
Ingénierie des Matériaux Polymères (IMP), UMR 5223, 15 bd Latarjet, F-69622 
Villeurbanne, France. Electronic address: laurent.david@univ-lyon1.fr.

In this study, a chitosan derivative with strong iron (III) chelating 
capabilities was developed by grafting the Deferoxamine (DFO) chelator to 
achieve a substitution degree of 3.8 ± 0.2 %. Through blending with ungrafted 
chitosan of low degree of acetylation (DA), a formulation able to form a 
physical hydrogels was formed in aqueous media, without the requirement of a 
cross-linking agent. The functionalization of chitosan with DFO led to xerogels 
exhibiting superior iron (III) chelation capacity and higher swelling when 
exposed to aqueous solutions, in comparison with to an unmodified chitosan 
xerogel. Notably, this material extracts iron (III) even against the strong iron 
chelator deferiprone. Furthermore, the material demonstrates selectivity for 
iron (III) chelation even in the presence of competing cations like copper (II) 
and zinc (II).

Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.

DOI: 10.1016/j.carbpol.2024.122670
PMID: 39486926 [Indexed for MEDLINE]

Conflict of interest statement: Declaration of competing interest The authors 
declare the following financial interests/personal relationships which may be 
considered as potential competing interests: LUX reports financial support was 
provided by Agence Nationale de la Recherche (ANR). DAVID, SALAZAR ARIZA, 
MONTEMBAULT, LUX, TILLEMENT has patent #FR2110474 pending to MEXBRAIN. DAVID 
MONTEMBAULT, LUX, TILLEMENT has patent #FR2007997 pending to MEXBRAIN. The other 
authors declare that they have no known competing financial interests or 
personal relationships that could have appeared to influence the work reported 
in this paper.